A novel NGS library preparation method to characterize native termini of fragmented DNA

A fast solution to NGS library preparation with low nanogram DNA input

Next-generation sequencing (NGS) has significantly impacted human genetics, enabling a comprehensive characterization of human genome as well as better understanding of many genomic abnormalities. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized medicine a reality in the foreseeable future. To date, library construction with clinical samples...

Automating FFPE Samples from Extraction to NGS Library Preparation

Building on our established automation and applications expertise across the genomics workflow, we describe an automated workflow partnering with Covaris®, Inc. to prepare NGS libraries from FFPE tissues samples (Figure 1). In this application note, we demonstrate an automated, high-throughput workflow that encompasses DNA extraction, purification, sample quality assessment, and subsequent libr...

Optimized NGS Library Preparation with Acoustic Liquid Handling APPLICATION NOTE

Radiant Genomics and Labcyte have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo® 525 Liquid Handler. In addition to cost savings, we demonstrate an increase in sample-processing throughput with the Echo system due to efficient multiplexing, which would otherwise remain impractical using standard liquid handling automation. In addi...

Beyond library size : a field guide to NGS normalization

A new method to prevent carry-over contaminations in two-step PCR NGS library preparations

Two-step PCR procedures are an efficient and well established way to generate amplicon libraries for NGS sequencing. However, there is a high risk of cross-contamination by carry-over of amplicons from first to second amplification rounds, potentially leading to severe misinterpretation of results. Here we describe a new method able to prevent and/or to identify carry-over contaminations by int...